This study was attempted to analysis whether there are any differences or not in the susceptibility to acute liver damage due to chemical hepato-toxic agents between young white rat and mature one.
The experiment was carried out in four divided groups as follows; control group, CCl(_4) group, chloroform group, and ethionine group. Each group was subdivided again into weight of 30gm, 50gm, 70gm, 150gm, 250gm and 300gm subgroups of both sexes respectively. 12 rats were studied in subgroup of normal control, whereas six rats alloted to each of experimenral subgroups and only male white rats were used in ethionine group.
The CCl(_4) group was subcutaneously injected with 0.2ml of CCl(_4) per 100gm of body weight, the Chloroform group similarly with 1.2ml of Chloroform per kg. and the ethionine group given with 75mg of ethionine per 100gm of weight a day, divided in three times, into the peritoneal cavity.
24 hours following injections as above, performing blood sampling through femoral vein, determination of serum transaminase and serum protein eletrophoresis was carried out, and after measuring weight of liver, tissue preparation with liver specimen for H-E stain, PAS stain and sudan Ⅲ stain was made respectively.
The results obtained are as follows;
1. In the CCl(_4) group, weight of liver was increased in subgroups of both sexes of 50gm, female subgroups of both sexes of 150gm and 250gm.
The serum G.O.T. and G.P.T. level were increased from 3 to 6 time and 9 to 12 times respectively in all subgroups of both sexes than normal control group.
The degree of hepato-lobular necrosis and fatty degeneration was observed to have only slight change in subgroups of both sexes of 30gm, 50gm and 70gm, whereas remarkable changes subgroups of 150gm, 250gm & 300gm.
2. In the chloroform group, remarkable weight gain of liver was noted in subgroups of both sexes of 70gm, 150gm, 250gm. An increament of serum G.O.T. level was found in subgroups of both sexes of 150gm and 250gm and 300gm. The value of serum G.P.T. was increased twice in subgroup of both sexes of 30gm, four times both in male subgroups of 150gm and in female subgroup of 70gm, and three times in subgroups of 250gm, than in normal control group. Severe degree of hepato-lobular necrosis as well as fatty degeneration was observed in subgroups of 70gm, 150gm, 250gm and 300gm, while moderate degree of changes detected in subgroups of both sexes of 30gm and 50gm.
3. In the ethionine group, weight gain of liver was observed gradually from subgroups of 70gm on, and went on more subgroups of 150gm, 250gm and 300gm. There was no significant increase in serum G.O.T. and G.P.T. value among the ethionine groups. The subgroup of 30gm and 50gm showed a mild degree of necrosis as well as degeneration was detected in subgroups of 70gm, 150gm, 250gm and 300gm.
Judging from the above results, 24 hours following injection of CCl(_4), chloroform and ethionine, acute liver damage observed among experimental groups was less pronounced in young white rat than in mature one.
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